Morphological and molecular analysis of a double-flowered mutant of the dioecious plant white campion showing both meristic and homeotic effects

Scutt, Charles P., Oliveira, Margarida, Gilmartin, Philip M. and Negrutiu, Ioan (1999) Morphological and molecular analysis of a double-flowered mutant of the dioecious plant white campion showing both meristic and homeotic effects. Developmental Genetics, 25 (3). pp. 267-279. ISSN 0192-253X

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Abstract

Many double-flowered plants, in which petals replace stamens, are highly valued by the horticultural industry. These mutants exhibit a homeotic conversion of floral organs and frequently also a meristic increase n floral organ number. By gamma irradiation we generated a novel double-flowered mutant, Sl-dfl, in a male genetic background of the dioecious plant white campion (Silene latifolia). This mutant shows a homeotic conversion of stamens to petals, together with uncontrolled growth and division of second and third whorl floral organ primordia, causing a proliferation of petal and chimeric petal-stamen organs. We characterize this mutant developmentally by scanning electron microscopy and demonstrate that the effects of the mutation commence following the formation of a correctly partitioned floral meristem with a wild-type arrangement of organ primordia. We have commenced a molecular investigation of the Sl-dfl mutant by testing the expression and genomic organization of the known white campion putative MADS-box floral homeotic genes. These studies indicate four MADS-box genes to be unlikely to be mutated in the double-flowered mutant. The possibility that a putative C- function MADS-box gene may cause the mutant phenotype has not currently been excluded, though our morphological studies suggest that a C-function mutation is not involved in this case. We conclude that a number of different classes of double-flowered mutation exist, not all of which are currently known from model plant species. This may be indicative of important developmental differences between species and may also emphasize a need for comparative studies of floral development.

Item Type: Article
Uncontrolled Keywords: double flower,silene latifolia,dioecy,mads-box,homeotic,meristic,secondary flower,gamma radiation
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: Pure Connector
Date Deposited: 01 Nov 2013 14:16
Last Modified: 20 May 2020 23:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/44154
DOI: 10.1002/(SICI)1520-6408(1999)25:3<267::AID-DVG9>3.0.CO;2-9

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