Insights into molecular recognition of Lewis(X) mimics by DC-SIGN using NMR and molecular modelling

Guzzi, Cinzia, Angulo, Jesús, Doro, Fabio, Reina, José J, Thépaut, Michel, Fieschi, Franck, Bernardi, Anna, Rojo, Javier and Nieto, Pedro M (2011) Insights into molecular recognition of Lewis(X) mimics by DC-SIGN using NMR and molecular modelling. Organic & Biomolecular Chemistry, 9 (22). pp. 7705-7712. ISSN 1477-0539

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Abstract

In this work, we have studied in detail the binding of two a-fucosylamide-based mimics of Lewis(X) to DC-SIGN ECD (ECD = extracellular domain) using STD NMR and docking. We have concluded that the binding mode occurs mainly through the fucose moiety, in the same way as Lewis(X). Similarly to other mimics containing mannose or fucose previously studied, we have shown that both compounds bind to DC-SIGN ECD in a multimodal fashion. In this case, the main contact is the interaction of two hydroxyl groups one equatorial and the other one axial (O3 and O4) of the fucose with the Ca(2+) as Lewis(X) and similarly to mannose-containing mimics (in this case the interacting groups are both in the equatorial position). Finally, we have measured the K(D) of one mimic that was 0.4 mM. Competitive STD NMR experiments indicate that the aromatic moiety provides additional binding contacts that increase the affinity.

Item Type: Article
Uncontrolled Keywords: dendritic cells,models, molecular,humans,mannose,fucose,biomimetics,immunity, innate,antigens, cd15,receptors, cell surface,protein binding,cell adhesion molecules,binding sites,magnetic resonance spectroscopy,small molecule libraries,lectins, c-type,kinetics,molecular sequence data,molecular mimicry,protein structure, tertiary,carbohydrate conformation
Faculty \ School: Faculty of Science > School of Pharmacy
Depositing User: Pure Connector
Date Deposited: 21 Oct 2013 19:30
Last Modified: 24 Apr 2020 23:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/43766
DOI: 10.1039/c1ob05938f

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