Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue

Canales, Angeles, Lozano, Rosa, López-Méndez, Blanca, Angulo, Jesús, Ojeda, Rafael, Nieto, Pedro M., Martín-Lomas, Manuel, Giménez-Gallego, Guillermo and Jiménez-Barbero, Jesús (2006) Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue. FEBS Journal, 273 (20). pp. 4716-4727. ISSN 1742-464X

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Abstract

The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity.

Item Type: Article
Faculty \ School: Faculty of Science > School of Pharmacy
Related URLs:
Depositing User: Pure Connector
Date Deposited: 21 Oct 2013 20:00
Last Modified: 17 Mar 2020 18:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/43757
DOI: 10.1111/j.1742-4658.2006.05474.x

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