Canonical Wnt signals combined with suppressed TGFβ/BMP pathways promote renewal of the native human colonic epithelium

Reynolds, Amy, Wharton, Natalia, Parris, Alyson, Mitchell, Esther, Sobolewski, Anastasia, Kam, Christy, Bigwood, Loren, El Hadi, Ahmed, Münsterberg, Andrea, Lewis, Michael, Speakman, Christopher, Stebbings, William, Wharton, Richard, Sargen, Kevin, Tighe, Richard, Jamieson, Crawford, Hernon, James, Kapur, Sandeep, Oue, Naohide, Yasui, Wataru and Williams, Mark (2014) Canonical Wnt signals combined with suppressed TGFβ/BMP pathways promote renewal of the native human colonic epithelium. Gut, 63 (4). pp. 610-621. ISSN 0017-5749

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Abstract

Background: A defining characteristic of the human intestinal epithelium is that it is the most rapidly renewing tissue in the body. However, the processes underlying tissue renewal and the mechanisms that govern their coordination have proved difficult to study in the human gut. Objective: To investigate the regulation of stem cell-driven tissue renewal by canonical Wnt and TGFβ/bone morphogenetic protein (BMP) pathways in the native human colonic epithelium. Design: Intact human colonic crypts were isolated from mucosal tissue samples and placed into 3D culture conditions optimised for steady-state tissue renewal. High affinity mRNA in situ hybridisation and immunohistochemistry were complemented by functional genomic and bioimaging techniques. The effects of signalling pathway modulators on the status of intestinal stem cell biology, crypt cell proliferation, migration, differentiation and shedding were determined. Results: Native human colonic crypts exhibited distinct activation profiles for canonical Wnt, TGFβ and BMP pathways. A population of intestinal LGR5/OLFM4-positive stem/progenitor cells were interspersed between goblet-like cells within the crypt-base. Exogenous and crypt cell-autonomous canonical Wnt signals supported homeostatic intestinal stem/progenitor cell proliferation and were antagonised by TGFβ or BMP pathway activation. Reduced Wnt stimulation impeded crypt cell proliferation, but crypt cell migration and shedding from the crypt surface were unaffected and resulted in diminished crypts. Conclusions: Steady-state tissue renewal in the native human colonic epithelium is dependent on canonical Wnt signals combined with suppressed TGFβ/BMP pathways. Stem/progenitor cell proliferation is uncoupled from crypt cell migration and shedding, and is required to constantly replenish the crypt cell population.

Item Type: Article
Additional Information: This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/
Uncontrolled Keywords: adult,aged,aged, 80 and over,bone morphogenetic proteins,cell differentiation,cell movement,cell proliferation,colon,humans,in situ hybridization,intestinal mucosa,microscopy, confocal,middle aged,regeneration,signal transduction,stem cells,transforming growth factor beta,wnt signaling pathway
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 20 Aug 2013 05:29
Last Modified: 24 Jun 2020 23:47
URI: https://ueaeprints.uea.ac.uk/id/eprint/43142
DOI: 10.1136/gutjnl-2012-304067

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