A novel co-operative mechanism linking TGFb and Lyn Kinase activation to imatinib resistance in chronic myeloid leukaemia cells

Smith, Paul G., Tanaka, Hideo and Chantry, Andrew (2012) A novel co-operative mechanism linking TGFb and Lyn Kinase activation to imatinib resistance in chronic myeloid leukaemia cells. Oncotarget, 3 (5). pp. 518-524. ISSN 1949-2553

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Abstract

The advent of a mechanism specific inhibitor imatinib, targeting Bcr-Abl kinase, has paved the way for new treatment strategies in chronic myeloid leukaemia (CML). However, resistance to imatinib is common in patients and has recently been linked to both transforming growth factor-β (TGFβ) and elevated Lyn kinase activity, although molecular mechanisms remain largely unknown. Here, using leukaemic MYL cell lines derived from CML patients, we show that TGFβ plays a key role in imatinib-resistance via direct effects on Lyn ubiquitination and turnover that results in bursts of Lyn kinase activity, and identify c-cbl is a candidate E3 ubiquitin ligase. Furthermore, blockade of TGFβ signalling activity with the TGFβ receptor kinase inhibitor SB431542 significantly reduces Lyn turnover and activation, and subsequently enhances imatinib-mediated CML cell death in a proteasomal-dependent manner. Collectively, our data reveals novel co-operative mechanisms in CML involving TGFβ and Lyn kinase linked to proteasome function and ubiquitination, and thus supports therapeutic approaches that target TGFβ pathway activity as a strategy for overcoming imatinib-resistance in CML.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 25 Jun 2013 12:50
Last Modified: 08 Nov 2022 09:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/42749
DOI: 10.18632/oncotarget.500

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