Trans-sialidase Stimulates Eat Me Response from Epithelial Cells

Butler, Claire E, de Carvalho, Tecia M U, Grisard, Edmundo C, Field, Robert A and Tyler, Kevin (2013) Trans-sialidase Stimulates Eat Me Response from Epithelial Cells. Traffic, 14 (7). pp. 853-869. ISSN 1600-0854

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Abstract

Epithelial cell invasion by the protozoan parasite Trypanosoma cruzi is enhanced by the presence of an enzyme expressed on its cell surface during the trypomastigote life cycle stage. The enzyme, trans-sialidase (TS), is a member of one of the largest gene families expressed by the parasite and the role of its activity in mediating epithelial cell entry has not hitherto been understood. Here we show that the T. cruzi TS generates an eat me signal which is capable of enabling epithelial cell entry. We have utilized purified, recombinant, active (TcTS) and inactive (TcTS2V0) TS coated onto beads to challenge an epithelial cell line. We find that TS activity acts upon G protein coupled receptors present at the epithelial cell synapse with the coated bead, thereby enhancing cell entry. By so doing, we provide evidence that TS proteins bind glycans, mediate the formation of distinct synaptic domains and promote macropinocytotic uptake of microparticles into a perinuclear compartment in a manner which may emulate entosis.

Item Type: Article
Additional Information: © 2013 The Authors. Traffic published by John Wiley & Sons Ltd.
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 26 Jun 2013 09:20
Last Modified: 21 Apr 2020 21:46
URI: https://ueaeprints.uea.ac.uk/id/eprint/42531
DOI: 10.1111/tra.12078

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