Use of the mouse aortic ring assay to study angiogenesis

Baker, Marianne, Robinson, Stephen, Lechertier, Tanguy, Barber, Paul R, Tavora, Bernardo, D'Amico, Gabriela, Jones, Dylan T, Vojnovic, Boris and Hodivala-Dilke, Kairbaan (2012) Use of the mouse aortic ring assay to study angiogenesis. Nature Protocols, 7 (1). pp. 89-104.

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Abstract

Here we provide a protocol for quantitative three-dimensional ex vivo mouse aortic ring angiogenesis assays, in which developing microvessels undergo many key features of angiogenesis over a timescale similar to that observed in vivo. The aortic ring assay allows analysis of cellular proliferation, migration, tube formation, microvessel branching, perivascular recruitment and remodeling-all without the need for cellular dissociation-thus providing a more complete picture of angiogenic processes compared with traditional cell-based assays. Our protocol can be applied to aortic rings from embryonic stage E18 through to adulthood and can incorporate genetic manipulation, treatment with growth factors, drugs or siRNA. This robust assay allows assessment of the salient steps in angiogenesis and quantification of the developing microvessels, and it can be used to identify new modulators of angiogenesis. The assay takes 6-14 d to complete, depending on the age of the mice, treatments applied and whether immunostaining is performed.

Item Type: Article
Uncontrolled Keywords: microscopy, fluorescence,cell movement,animals,microscopy, phase-contrast,neovascularization, physiologic,aorta,tissue culture techniques,mice, inbred c57bl,mice,cell proliferation
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 22 Aug 2013 05:24
Last Modified: 21 Apr 2020 21:45
URI: https://ueaeprints.uea.ac.uk/id/eprint/42485
DOI: 10.1038/nprot.2011.435

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