Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat

McGuinness, Sheila, McCabe, Evonne, O’Regan, Edel, Dolan, Anthony, Duffy, Geraldine, Burgess, Catherine, Fanning, Seamus, Barry, Thomas and O'Grady, Justin (2009) Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat. Meat Science, 83 (3). pp. 555-562. ISSN 1873-4138

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Abstract

In this study, a combined enrichment/real-time PCR method for the rapid detection of Salmonella on fresh meat carcasses, was designed, developed and validated in-house following requirements outlined in ISO 16140:2003. The method included an 18 h non-selective enrichment in buffered peptone water (BPW) and a 6 h selective enrichment in Rappaport Vasilliadis Soya (RVS) broth, based on the traditional culture method, ISO 6579:2002. The real-time PCR assay included an internal amplification control (IAC), was 100% specific and was sensitive to one cell equivalent. The alternative method was validated against the traditional culture method and relative accuracy of 94.9%, sensitivity of 94.7% and specificity of 100% were determined using 150 fresh meat carcass swabs. This alternative method had a detection limit of 1–10 CFU/100 cm2 for fresh meat carcass swabs and was performed in 26 h. Following further inter-laboratory studies, this alternative method could be suitable for implementation in testing laboratories for the analysis of carcass swabs.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018)
Depositing User:	Sophie Buckingham
Date Deposited:	05 Mar 2013 10:29
Last Modified:	28 Mar 2025 03:22
URI:	https://ueaeprints.uea.ac.uk/id/eprint/41783
DOI:	10.1016/j.meatsci.2009.07.004

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