Efficacies of calcium-EDTA in combination with imipenem in a murine model of sepsis caused by Escherichia coli with NDM-1 β-lactamase

Yoshizumi, Ayumi, Ishii, Yoshikazu, Livermore, David M, Woodford, Neil, Kimura, Soichiro, Saga, Tomoo, Harada, Sohei, Yamaguchi, Keizo and Tateda, Kazuhiro (2013) Efficacies of calcium-EDTA in combination with imipenem in a murine model of sepsis caused by Escherichia coli with NDM-1 β-lactamase. Journal of Infection and Chemotherapy, 19 (5). pp. 992-5. ISSN 1341-321X

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Abstract

We evaluated the efficacy of ethylenediamine-N,N,N',N'-tetraacetic acid, disodium calcium salt (Ca-EDTA), as an inhibitor for New Delhi metallo-β-lactamase-1 (NDM-1) in vitro antibiotic susceptibility and in a mouse model of sepsis caused by Escherichia coli. Ca-EDTA drastically reduced the MICs of carbapenems for all NDM-producing bacteria [imipenem (IPM) ≤1-2 μg/ml; meropenem (MEPM) ≤1-4 µg/ml]. In the neutropenic murine model of sepsis, the bacterial burden was further reduced by combination therapy using imipenem/cilastatin sodium (IPM/CS) and Ca-EDTA to 2.3 × 10(3) CFU/liver, compared with 2.9 × 10(4) CFU/liver for IPM/CS alone. These data demonstrated the possibility of Ca-EDTA for clinical applications. In our understanding, this is the first report examining the effect of Ca-EDTA on a mouse sepsis model caused by NDM-1-producing bacteria.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Sophie Buckingham
Date Deposited: 05 Mar 2013 10:38
Last Modified: 21 Apr 2020 16:12
URI: https://ueaeprints.uea.ac.uk/id/eprint/41725
DOI: 10.1007/s10156-012-0528-y

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