Davidson, M. G., Wormstone, I. M. ORCID: https://orcid.org/0000-0002-6423-7766, Morgan, D., Malakof, R., Allen, J. and McGahan, M. C.
(2000)
Ex vivo canine lens capsular sac explants.
Graefe's Archive for Clinical and Experimental Ophthalmology, 238 (8).
pp. 708-714.
ISSN 0721-832X
Abstract
Background: Lens capsular sac explants from human cadaver eyes were used to investigate posterior capsular opacification (PCO). The purpose of this study was to characterize a similar model using canine tissue and to determine whether transferrin (Tf), transforming growth factor β-2 (TGF-β2), and insulin-like growth factor-1 (IGF-1) are secreted by lens epithelial cells (LEC) of these ex vivo sacs. Methods: The lens from canine eyes was removed by extracapsular cataract extraction, the lens sac dissected free, pinned to a petri dish, and cultured in either serum-supplemented or serum-free medium. Morphologic characteristics and growth rate to confluence on the posterior capsule were studied by phase-contrast microscopy. Vimentin, alpha smooth muscle actin, and panTGF-β expression by LEC were determined by immunohistochemistry. Tf, TGF-β2, and IGF-1 levels were measured by ELISA in the supernatant of sacs cultured in serum-free medium. Results: The mean time to confluence of LEC onto the posterior capsule was 5.4±1.1 days (n=22) and 14.7±3.7 days (n=14) for sacs in serum-supplemented and serum-free medium, respectively. Following development of confluence, explants displayed opacification and light scatter from cellular proliferation and capsular contraction. Confluent LEC expressed vimentin, alpha smooth muscle actin, and TGF-β2, and both Tf and TGF-β2 were secreted into the culture supernatant. Conclusion: Canine lens sac explants have characteristics virtually identical to those of human origin, and appear to be a useful alternative tissue source for this model when human cadaver eyes are unavailable. Tf and TGFβ-2, but not IGF-1, are secreted by LEC in explanted lens sacs and may influence the proliferation and metaplasia of LEC during the development of PCO.
Item Type: | Article |
---|---|
Faculty \ School: | Faculty of Science > School of Biological Sciences |
UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
Depositing User: | Users 2731 not found. |
Date Deposited: | 04 Dec 2012 12:52 |
Last Modified: | 23 Jul 2024 15:36 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/40356 |
DOI: | 10.1007/s004170000158 |
Actions (login required)
![]() |
View Item |