Links between methanotroph community composition and CH4 oxidation in a pine forest soil

Bengtson, Per, Basiliko, Nathan, Dumont, Marc G., Hills, Melissa, Murrell, J. Colin, Grayston, Sue J. and Roy, Réal (2009) Links between methanotroph community composition and CH4 oxidation in a pine forest soil. FEMS Microbiology Ecology, 70 (3). pp. 356-366. ISSN 1574-6941

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The main gap in our knowledge about what determines the rate of CH4 oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following 13CH4 incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH4 oxidation rates. The rates of 13C incorporation into PLFAs differed between horizons, with low 13C incorporation in the organic soil and relatively high 13C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the 13C label also differed between horizons, and statistical analyses suggested that the methanotroph community composition was a major cause of variation in CH4 oxidation rates. Both PLFA and pmoA-based data indicated that CH4 oxidizers in this soil belong to the uncultivated ‘upland soil cluster a’. CH4 oxidation potential exhibited the opposite pattern to 13C incorporation, suggesting that CH4 oxidation potential assays may correlate poorly with in situ oxidation rates. The DNA/RNA-SIP assay was not successful, most likely due to insufficient 13C-incorporation into DNA/RNA. The limitations of the technique are briefly discussed.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Environmental Biology
Depositing User: Rhiannon Harvey
Date Deposited: 27 Mar 2012 14:11
Last Modified: 20 Mar 2023 15:31
DOI: 10.1111/j.1574-6941.2009.00751.x

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