Response to mercury (II) ions in Methylococcus capsulatus (Bath)

Boden, R and Murrell, JC (2011) Response to mercury (II) ions in Methylococcus capsulatus (Bath). FEMS Microbiology Letters, 324 (2). pp. 106-110. ISSN 0378-1097

Full text not available from this repository. (Request a copy)

Abstract

The mercury (II) ion is toxic and is usually detoxified in Bacteria by reduction to elemental mercury, which is less toxic. This is catalysed by an NAD(P)H-dependent mercuric reductase (EC 1.16.1.1). Here, we present strong evidence that Methylococcus capsulatus (Bath) – a methanotrophic member of the Gammaproteobacteria – uses this enzyme to detoxify mercury. In radiorespirometry studies, it was found that cells exposed to mercury dissimilated 100% of [14C]-methane provided to generate reducing equivalents to fuel mercury (II) reduction, rather than the mix of assimilation and dissimilation found in control incubations. The detoxification system is constitutively expressed with a specific activity of 352 (±18) nmol NADH oxidized min-1 (mg protein)-1. Putative mercuric reductase genes were predicted in the M. capsulatus (Bath) genome and found in mRNA microarray studies. The MerA-derived polypeptide showed high identity (> 80%) with MerA sequences from the Betaproteobacteria.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: Rhiannon Harvey
Date Deposited: 27 Mar 2012 09:43
Last Modified: 24 Jul 2019 13:21
URI: https://ueaeprints.uea.ac.uk/id/eprint/38482
DOI: 10.1111/j.1574-6968.2011.02395.x

Actions (login required)

View Item View Item