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ToolsRunguphan, Weerawat, Qu, Xudong and O'Connor, Sarah E. (2010) Integrating carbon–halogen bond formation into medicinal plant metabolism. Nature, 468 (7322). pp. 461-464. ISSN 0028-0836
Full text not available from this repository.Abstract
Halogenation, once considered a rare occurrence in nature, has now been observed in many natural product biosynthetic pathways1. However, only a small fraction of halogenated compounds have been isolated from terrestrial plants2. Given the impact that halogenation can have on the biological activity of natural products1, we rationalized that introduction of halides into medicinal plant metabolism would provide the opportunity to rationally bioengineer a broad variety of novel plant products with altered, and perhaps improved, pharmacological properties. Here we report that chlorination biosynthetic machinery from soil bacteria can be successfully introduced into the medicinal plant Catharanthus roseus (Madagascar periwinkle). These prokaryotic halogenases function within the context of the plant cell to generate chlorinated tryptophan, which is then shuttled into monoterpene indole alkaloid metabolism to yield chlorinated alkaloids. A new functional group– a halide– is thereby introduced into the complex metabolism of C. roseus, and is incorporated in a predictable and regioselective manner onto the plant alkaloid products. Medicinal plants, despite their genetic and developmental complexity, therefore appear to be a viable platform for synthetic biology efforts.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry |
| UEA Research Groups: | Faculty of Science > Research Groups > Synthetic Chemistry (former - to 2017) |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 21 Mar 2012 13:40 |
| Last Modified: | 10 Mar 2024 00:49 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/38398 |
| DOI: | 10.1038/nature09524 |
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