A fully human in vitro capsular bag model to permit intraocular lens evaluation

Dawes, Lucy J., Illingworth, Christopher D. and Wormstone, I. Michael ORCID: https://orcid.org/0000-0002-6423-7766 (2012) A fully human in vitro capsular bag model to permit intraocular lens evaluation. Investigative Ophthalmology & Visual Science, 53 (1). pp. 23-29. ISSN 1552-5783

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Purpose. To establish a fully human in vitro culture model with which to test the putative effects of intraocular lens (IOL) designs in preventing posterior capsule opacification (PCO) after cataract surgery. Methods. A sham cataract operation was performed to prepare human capsular bags from donor lenses. In one capsular bag of a donor pair, an intraocular lens (PMMA round-edge IOL or acrylic IOL) was implanted while the other capsular bag remained aphakic. Bags were transferred to a Petri dish and secured anterior-face down using entomological pins. Capsular bags were maintained in Eagle's minimum essential medium supplemented with 2% human serum and 10 ng/mL TGF-ß to drive growth and matrix contraction. Results. In the absence of an IOL, cells appeared within the central posterior capsule at 4.38 ± 0.26 days, whereas in the presence of a PMMA round-edge IOL or an acrylic IOL they appeared at 8 ± 0.41 days and 11 ± 0.7 days, respectively. Immunocytochemical analysis showed an accumulation of cells at the edge of the acrylic IOL and a less evident accumulation with the PMMA round-edge IOL. Moreover, matrix contraction was more prominent in the absence of an IOL but was still apparent, to a lesser degree, in the presence of a PMMA round-edge IOL. The acrylic IOL greatly suppressed matrix contraction. Conclusions. The authors have developed a fully human in vitro capsular bag system that relates well to clinical observations and permits the testing of novel intraocular lenses.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Cells and Tissues
Depositing User: Users 2731 not found.
Date Deposited: 27 Feb 2012 12:06
Last Modified: 21 Apr 2023 23:39
URI: https://ueaeprints.uea.ac.uk/id/eprint/37518
DOI: 10.1167/iovs.11-8851

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