The Structure of an Open Form of an E. coli Mechanosensitive Channel at 3.45 A Resolution

Wang, Wenjian, Black, Susan S., Edwards, Michelle D., Miller, Samantha, Morrison, Emma L., Bartlett, Wendy, Dong, Changjiang, Naismith, James H. and Booth, Ian R. (2008) The Structure of an Open Form of an E. coli Mechanosensitive Channel at 3.45 A Resolution. Science, 321 (5893). pp. 1179-1183. ISSN 0036-8075

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Abstract

How ion channels are gated to regulate ion flux in and out of cells is the subject of intense interest. The Escherichia coli mechanosensitive channel, MscS, opens to allow rapid ion efflux, relieving the turgor pressure that would otherwise destroy the cell. We present a 3.45 angstrom-resolution structure for the MscS channel in an open conformation. This structure has a pore diameter of ~13 angstroms created by substantial rotational rearrangement of the three transmembrane helices. The structure suggests a molecular mechanism that underlies MscS gating and its decay of conductivity during prolonged activation. Support for this mechanism is provided by single-channel analysis of mutants with altered gating characteristics.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Rhiannon Harvey
Date Deposited: 22 Feb 2012 10:20
Last Modified: 21 Apr 2020 17:51
URI: https://ueaeprints.uea.ac.uk/id/eprint/37347
DOI: 10.1126/science.1159262

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