Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity

Nekrasov, Vladimir, Li, Jing, Batoux, Martine, Roux, Milena, Chu, Zhao-Hui, Lacombe, Severine, Rougon, Alejandra, Bittel, Pascal, Kiss-Papp, Marta, Chinchilla, Delphine, van Esse, H Peter, Jorda, Lucia, Schwessinger, Benjamin, Nicaise, Valerie, Thomma, Bart P H J, Molina, Antonio, Jones, Jonathan D G and Zipfel, Cyril (2009) Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity. The EMBO Journal, 28 (21). pp. 3428-3438. ISSN 0261-4189

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Abstract

In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.

Item Type: Article
Uncontrolled Keywords: arabidopsis,arabidopsis proteins,carrier proteins,endoplasmic reticulum,hsp40 heat-shock proteins,immunity, innate,plant diseases,receptors, pattern recognition
Faculty \ School: Faculty of Science > School of Biological Sciences

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Date Deposited: 21 Feb 2012 16:23
Last Modified: 21 Apr 2020 17:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/37341
DOI: 10.1038/emboj.2009.262

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