NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant

Hall, Lindsay J. ORCID: https://orcid.org/0000-0001-8938-5709, Clare, Simon and Dougan, Gordon (2010) NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant. Journal of Immunology, 184 (8). pp. 4327-4337. ISSN 0022-1767

Full text not available from this repository. (Request a copy)

Abstract

NK cells were found to be recruited in a temporally controlled manner to the nasal-associated lymphoid tissue and the cervical lymph nodes of mice after intranasal immunization with Ag85B-early secreted antigenic target 6kDa from Mycobacterium tuberculosis mixed with Escherichia coli heat-labile toxin as adjuvant. These NK cells were activated and secreted a diverse range of cytokines and other immunomodulators. Using Ab depletion targeting anti-asialo GM1, we found evidence for altered trafficking, impaired activation, and cytokine secretion of dendritic cells, macrophages, and neutrophils in immunized NK cell-depleted mice compared with control animals. Analysis of Ag-specific immune responses revealed an attenuated Ab and cytokine response in immunized NK cell-depleted animals. Systemic administration of rIL-6 but not rIFN-γ significantly restored immune responses in mice depleted of NK cells. In conclusion, cytokine production, particularly IL-6, via NK cells and NK cell-activated immune populations plays an important role in the establishment of local innate immune responses and the consequent development of adaptive immunity after mucosal immunization.

Item Type:	Article
Additional Information:	A correction has been published: Correction: NK Cells Influence Both Innate and Adaptive Immune Responses after Mucosal Immunization with Antigen and Mucosal Adjuvant. J Immunol (2018) 201 (1): 306. DOI: 10.4049/jimmunol.0903357 Supplemental Table I was inadvertently omitted from the supplemental material when the article was published online. Supplemental Table I has now been added to the supplemental material that appears online.
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Users 2731 not found.
Date Deposited:	08 Feb 2012 11:53
Last Modified:	15 Sep 2023 14:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/36939
DOI:	10.4049/jimmunol.0903357

Actions (login required)

View Item