NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant

Hall, Lindsay J., Clare, Simon and Dougan, Gordon (2010) NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant. Journal of Immunology, 184 (8). pp. 4327-4337. ISSN 0022-1767

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Abstract

NK cells were found to be recruited in a temporally controlled manner to the nasal-associated lymphoid tissue and the cervical lymph nodes of mice after intranasal immunization with Ag85B-early secreted antigenic target 6kDa from Mycobacterium tuberculosis mixed with Escherichia coli heat-labile toxin as adjuvant. These NK cells were activated and secreted a diverse range of cytokines and other immunomodulators. Using Ab depletion targeting anti-asialo GM1, we found evidence for altered trafficking, impaired activation, and cytokine secretion of dendritic cells, macrophages, and neutrophils in immunized NK cell-depleted mice compared with control animals. Analysis of Ag-specific immune responses revealed an attenuated Ab and cytokine response in immunized NK cell-depleted animals. Systemic administration of rIL-6 but not rIFN-γ significantly restored immune responses in mice depleted of NK cells. In conclusion, cytokine production, particularly IL-6, via NK cells and NK cell-activated immune populations plays an important role in the establishment of local innate immune responses and the consequent development of adaptive immunity after mucosal immunization.

Item Type:	Article
Additional Information:	A correction has been published: Correction: NK Cells Influence Both Innate and Adaptive Immune Responses after Mucosal Immunization with Antigen and Mucosal Adjuvant. J Immunol (2018) 201 (1): 306. DOI: 10.4049/jimmunol.0903357 Supplemental Table I was inadvertently omitted from the supplemental material when the article was published online. Supplemental Table I has now been added to the supplemental material that appears online.
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Users 2731 not found.
Date Deposited:	08 Feb 2012 11:53
Last Modified:	29 Mar 2025 00:59
URI:	https://ueaeprints.uea.ac.uk/id/eprint/36939
DOI:	10.4049/jimmunol.0903357

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