Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis.

Balk, J, Pierik, A J, Aguilar Netz, D J, Mühlenhoff, U and Lill, R (2005) Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis. Biochemical Society Transactions, 33 (Pt 1). pp. 86-89. ISSN 0300-5127

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Abstract

The genome of the yeast Saccharomyces cerevisiae encodes the essential protein Nar1p that is conserved in virtually all eukaryotes and exhibits striking sequence similarity to bacterial iron-only hydrogenases. Previously, we have shown that Nar1p is an Fe-S protein and that assembly of its co-factors depends on the mitochondrial Fe-S cluster biosynthesis apparatus. Using functional studies in vivo, we demonstrated that Nar1p has an essential role in the maturation of cytosolic and nuclear, but not of mitochondrial, Fe-S proteins. Here we provide further spectroscopic evidence that Nar1p possesses two Fe-S clusters. We also show that Nar1p is required for Fe-S cluster assembly on the P-loop NTPase Nbp35p, another newly identified component of the cytosolic Fe-S protein assembly machinery. These data suggest a complex biochemical pathway of extra-mitochondrial Fe-S protein biogenesis involving unique eukaryotic proteins.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Rhiannon Harvey
Date Deposited: 07 Feb 2012 15:51
Last Modified: 21 Apr 2020 19:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/36917
DOI:

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