When metagenomics meets stable-isotope probing: progress and perspectives

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Chen, Y and Murrell, JC (2010) When metagenomics meets stable-isotope probing: progress and perspectives. Trends in Microbiology, 18 (4). pp. 157-163. ISSN 1878-4380

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Abstract

The application of metagenomics, the culture-independent capture and subsequent analysis of genomic DNA from the environment, has greatly expanded our knowledge of the diversity of microbes and microbial protein families; however, the metabolic functions of many microorganisms remain largely unknown. DNA stable-isotope probing (DNA-SIP) is a recently developed method in which the incorporation of stable isotope from a labelled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Environmental Sciences
Related URLs:	http://dx.doi.org/10.1016/j.tim.2010.02....
Depositing User:	Users 2731 not found.
Date Deposited:	23 Jan 2012 16:43
Last Modified:	23 Oct 2022 01:16
URI:	https://ueaeprints.uea.ac.uk/id/eprint/36375
DOI:	10.1016/j.tim.2010.02.002

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