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Bradley, Justin M., Silkstone, Gary, Wilson, Michael T., Cheesman, Myles R. and Butt, Julea N. 
ORCID: https://orcid.org/0000-0002-9624-5226
(2011)
Probing a complex of cytochromecand cardiolipin by magnetic circular dichroism spectroscopy: Implications for the initial events in apoptosis.
Journal of the American Chemical Society, 133 (49).
pp. 19676-19679.
ISSN 0002-7863
Abstract
Oxidation of cardiolipin (CL) by its complex with cytochrome c (cyt c) plays a crucial role in triggering apoptosis. Through a combination of magnetic circular dichroism spectroscopy and potentiometric titrations, we show that both the ferric and ferrous forms of the heme group of a CL:cyt c complex exist as multiple conformers at a physiologically relevant pH of 7.4. For the ferric state, these conformers are His/Lys- and His/OH–-ligated. The ferrous state is predominantly high-spin and, most likely, His/–. Interconversion of the ferric and ferrous conformers is described by a single midpoint potential of -80 ± 9 mV vs SHE. These results suggest that CL oxidation in mitochondria could occur by the reaction of molecular oxygen with the ferrous CL:cyt c complex in addition to the well-described reaction of peroxides with the ferric form.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry Faculty of Science > School of Biological Sciences |
| Depositing User: | Users 2731 not found. |
| Date Deposited: | 10 Jan 2012 14:32 |
| Last Modified: | 20 Dec 2022 14:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/36053 |
| DOI: | 10.1021/ja209144h |
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