A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation

Zafrilla, Basilio, Martínez‑Espinosa, Rosa María, Bonete, Mari­a José, Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226, Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Gates, Andrew J. ORCID: https://orcid.org/0000-0002-4594-5038 (2011) A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation. Biochemical Society Transactions, 39 (6). pp. 1844-1848. ISSN 0300-5127

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Abstract

In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3-/NO2- reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in the electron transfer cascade required for assimilatory nitrate and nitrite reduction by the cytoplasmic NarB- and NirA-type reductases respectively. UV–visible absorbance and EPR spectroscopic characterization of purified Hv-Fd demonstrate that this protein binds a single [2Fe–2S] cluster, and potentiometric titration reveals that the cluster shares similar redox properties with those present in plant-type ferredoxins.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry
Depositing User:	Users 2731 not found.
Date Deposited:	09 Jan 2012 16:41
Last Modified:	20 Dec 2022 10:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/35996
DOI:	10.1042/BST20110709

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