A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation
Zafrilla, Basilio, Martínez‑Espinosa, Rosa María, Bonete, Maria José, Butt, Julea N., Richardson, David J. and Gates, Andrew J. (2011) A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation. Biochemical Society Transactions, 39 (6). pp. 1844-1848. ISSN 0300-5127
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Abstract
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3-/NO2- reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in the electron transfer cascade required for assimilatory nitrate and nitrite reduction by the cytoplasmic NarB- and NirA-type reductases respectively. UV–visible absorbance and EPR spectroscopic characterization of purified Hv-Fd demonstrate that this protein binds a single [2Fe–2S] cluster, and potentiometric titration reveals that the cluster shares similar redox properties with those present in plant-type ferredoxins.
Item Type: | Article |
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Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry |
Depositing User: | Users 2731 not found. |
Date Deposited: | 09 Jan 2012 16:41 |
Last Modified: | 09 Jun 2022 13:45 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/35996 |
DOI: | 10.1042/BST20110709 |
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