Electrochemical titrations and reaction time courses monitored in situ by magnetic circular dichroism spectroscopy

Bradley, Jenna, Butt, Julea and Cheesman, Myles (2011) Electrochemical titrations and reaction time courses monitored in situ by magnetic circular dichroism spectroscopy. Analytical Biochemistry, 419 (2). pp. 110-116. ISSN 1096-0309

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Abstract

Magnetic circular dichroism (MCD) spectra, at ultraviolet–visible or near-infrared wavelengths (185–2000 nm), contain the same transitions observed in conventional absorbance spectroscopy, but their bisignate nature and more stringent selection rules provide greatly enhanced resolution. Thus, they have proved to be invaluable in the study of many transition metal-containing proteins. For mainly technical reasons, MCD has been limited almost exclusively to the measurement of static samples. But the ability to employ the resolving power of MCD to follow changes at transition metal sites would be a potentially significant advance. We describe here the development of a cuvette holder that allows reagent injection and sample mixing within the 50-mm-diameter ambient temperature bore of an energized superconducting solenoid. This has allowed us, for the first time, to monitor time-resolved MCD resulting from in situ chemical manipulation of a metalloprotein sample. Furthermore, we report the parallel development of an electrochemical cell using a three-electrode configuration with physically separated working and counter electrodes, allowing true potentiometric titration to be performed within the bore of the MCD solenoid.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Pharmacy
Faculty of Science > School of Chemistry
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Depositing User: Users 2731 not found.
Date Deposited: 31 Oct 2011 14:32
Last Modified: 17 Sep 2020 23:29
URI: https://ueaeprints.uea.ac.uk/id/eprint/35318
DOI: 10.1016/j.ab.2011.07.030

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