Time-resolved emission spectra of green fluorescent protein

Jaye, Andrew A., Stoner-Ma, Deborah, Matousek, Pavel, Towrie, Michael, Tonge, Peter J. and Meech, Stephen R. ORCID: https://orcid.org/0000-0001-5561-2782 (2006) Time-resolved emission spectra of green fluorescent protein. Photochemistry and Photobiology, 82 (2). pp. 373-379. ISSN 0031-8655

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The time-resolved emission spectra of wild-type green fluorescent protein (wtGFP) and the T203V GFP mutant have been recorded with picosecond time resolution, allowing the separate characterization of the two spectral components associated with the neutral and anionic forms of the GFP chromophore. Significantly, neither component shifts as a function of time. It is suggested that the absence of spectral shift is a result of highly restricted movement of the protein residues in the vicinity of the chromophore. The shapes of the separated spectra are discussed and their relative ratio analyzed in a steady-state analysis.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017)
Depositing User: Rachel Smith
Date Deposited: 26 Jul 2011 15:13
Last Modified: 29 Aug 2023 14:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/34190
DOI: 10.1562/2005-05-07-RA-518

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