Excited-state dynamics in the green fluorescent protein chromophore

Mandal, Debabrata, Tahara, Tahei and Meech, Stephen R. ORCID: https://orcid.org/0000-0001-5561-2782 (2004) Excited-state dynamics in the green fluorescent protein chromophore. The Journal of Physical Chemistry B, 108 (3). pp. 1102-1108. ISSN 1520-6106

Full text not available from this repository. (Request a copy)


The remarkable suppression of radiationless decay by the green fluorescent protein (GFP) is investigated through ultrafast fluorescence spectroscopy of its isolated chromophore in solution. Decay data are measured by fluorescence up-conversion as a function of solvent and wavelength for both neutral and anionic forms of the chromophore. All fluorescence decays are found to be well described by two exponentially decaying components. The effect of medium viscosity is slight, suggesting that the intramolecular motion promoting radiationless decay is a volume-conserving one. A minor effect of solvent polarity and H-bonding ability on the decay times is observed. The two decay constants are independent of emission wavelength, but their relative weights are not. Time-resolved fluorescence spectroscopy shows that the Stokes shift is complete in

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Depositing User: Rachel Smith
Date Deposited: 26 Jul 2011 15:19
Last Modified: 09 Feb 2023 13:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/34182
DOI: 10.1021/jp035816b

Actions (login required)

View Item View Item