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Kügler, Silke, Schuller, Stephanie 
ORCID: https://orcid.org/0000-0003-3260-9112 and Goebel, Werner
(1997)
Involvement of MAP-kinases and -phosphatases in uptake and intracellular replication of Listeria monocytogenes in J774 macrophage cells.
FEMS Microbiology Letters, 157 (1).
pp. 131-136.
ISSN 1574-6968
Abstract
In this study we show that protein tyrosine kinases and also protein tyrosine phosphatases are involved in the uptake of Listeria monocytogenes by J774 macrophages to a different extent than in the uptake of inert latex beads. In addition, protein tyrosine kinases are necessary for the intracellular growth and survival of L. monocytogenes. The expression of the MAP kinase phosphatase MKP-1, a protein tyrosine phosphatase, is induced upon infection, and phagocytosis of L. monocytogenes by J774 cells overexpressing the MKP-1 protein is reduced compared to control cells. The decreased phagocytosis of L. monocytogenes as a result of the MKP-1 overexpression in J774 macrophages suggests that the activation of the MAP kinase(s) ERK-1 and/or ERK-2 is an essential requirement for the uptake of L. monocytogenes by J774 macrophages.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 20 Jul 2011 10:24 |
| Last Modified: | 15 Dec 2022 02:02 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33979 |
| DOI: | 10.1111/j.1574-6968.1997.tb12763.x |
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