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Garmendia, Junkal, Phillips, Alan D., Carlier, Marie-France, Chong, Yuwen, Schuller, Stephanie 
ORCID: https://orcid.org/0000-0003-3260-9112, Marches, Olivier, Dahan, Sivan, Oswald, Eric, Shaw, Rob K., Knutton, Stuart and Frankel, Gad
(2004)
TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton.
Cellular Microbiology, 6 (12).
pp. 1167-1183.
ISSN 1462-5814
Abstract
Subversion of host cell actin microfilaments is the hallmark of enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli infections. Both pathogens translocate the trans-membrane receptor protein – translocated intimin receptor (Tir), which links the extracellular bacterium to the cell cytoskeleton. While both converge on neural Wiskott–Aldrich syndrome protein (N-WASP), Tir-mediated actin accretion by EPEC and EHEC differ in that TirEPEC requires both tyrosine phosphorylation and the host adaptor protein Nck, whereas TirEHEC is not phosphorylated and utilizes an unidentified linker. Here we report the identification of Tir-cytoskeleton coupling protein (TccP), a novel EHEC effector that displays an Nck-like coupling activity following translocation into host cells. A tccP mutant did not affect Tir translocation and focusing but failed to recruit α-actinin, Arp3, N-WASP and actin to the site of bacterial adhesion. When expressed in EPEC, bacterial-derived TccP restored actin polymerization activity following infection of an Nck-deficient cell line. TccP has a similar biological activity on infected human intestinal explants ex vivo. Purified TccP activates N-WASP stimulating, in the presence of Arp2/3, actin polymerization in vitro. These results show that EHEC translocates both its own receptor (Tir) and an Nck-like protein (TccP) to facilitate actin polymerization.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 20 Jul 2011 09:34 |
| Last Modified: | 02 Mar 2023 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33956 |
| DOI: | 10.1111/j.1462-5822.2004.00459.x |
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