Evidence for the opposing roles of different γδ T cell subsets in macrophage homeostasis

Tramonti, Daniela, Andrew, Elizabeth M, Rhodes, Kate, Newton, Darren J and Carding, Simon R (2006) Evidence for the opposing roles of different γδ T cell subsets in macrophage homeostasis. European Journal of Immunology, 36 (7). pp. 1729-1738. ISSN 0014-2980

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To ensure invading pathogens are eliminated with minimal damage to host tissues it is essential that macrophage activation be tightly regulated. Previously we demonstrated that a subset of gammadelta T cells (Vgamma1(+)) contributes to resolving pathogen-induced immune responses by killing activated macrophages. However, the exaggerated macrophage response seen in infected Vgamma1(+) T cell-deficient mice suggests that gammadelta T cells play a broader role in macrophage homeostasis and other subsets might promote macrophage activation. Using a macrophage:gammadelta T cell co-culture system we have shown that gammadelta T cells increase the activity of macrophages activated in vivo by Listeria monocytogenes infection. In a dose-dependent manner, gammadelta T cells up-regulated production of cytokines (TNF-alpha, IL-6, IL-10) and chemokines (MIP-1alpha, MIP-1beta) by Listeria-elicited macrophages. The ability to increase macrophage cytokine production was prominent among Vgamma4(+) gammadelta T cells. Reciprocally, Vgamma4(+) gammadelta T cells were activated by Listeria-elicited macrophages, resulting in production of the anti-inflammatory cytokine, IL-10. gammadelta T cell adoptive transfer experiments showed that Vgamma4(+) T cells protected TCRdelta(-/-) mice against Listeria-induced liver injury and necrosis. These findings identify distinct and non-overlapping roles for gammadelta T cell subsets in regulating macrophage function during pathogen-induced immune responses.

Item Type: Article
Uncontrolled Keywords: animals,cells, cultured,chemokines,coculture techniques,dose-response relationship, immunologic,homeostasis,listeria monocytogenes,macrophages,mice,mice, inbred c57bl,mice, knockout,receptors, antigen, t-cell, gamma-delta,t-lymphocyte subsets
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User: Rhiannon Harvey
Date Deposited: 14 Jul 2011 11:40
Last Modified: 07 Feb 2023 15:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/33730
DOI: 10.1002/eji.200635959

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