Isolation of fluoroquinolone-resistant O25b:H4-ST131 Escherichia coli with CTX-M-14 extended-spectrum -lactamase from UK river water

Dhanji, Hiran, Murphy, Niamh M, Akhigbe, Christine, Doumith, Michel, Hope, Russell, Livermore, David M and Woodford, Neil (2011) Isolation of fluoroquinolone-resistant O25b:H4-ST131 Escherichia coli with CTX-M-14 extended-spectrum -lactamase from UK river water. Journal of Antimicrobial Chemotherapy, 66 (3). pp. 512-516. ISSN 0305-7453

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Abstract

Objectives We analysed water sampled from the River Thames in London for Escherichia coli resistant to oxyimino-cephalosporins and/or fluoroquinolones, particularly seeking isolates with CTX-M extended-spectrum ß-lactamases (ESBLs) and members of the clinically important O25b:H4-ST131 lineage. Methods River water was collected from three urban sites on the River Thames by the City of London Port Health Authority on two occasions 1 week apart. Coliforms and E. coli were identified by the Quanti-Tray™ method. Disc susceptibility tests were performed and MICs were determined for E. coli isolates resistant to either ciprofloxacin or cefpodoxime and genetic relatedness was determined by PFGE and real-time PCR. PCR was used for phylogenetic and plasmid typing, to detect antibiotic resistance genes and to detect ISEcp1 upstream of blaCTX-M genes. blaCTX-M alleles were identified by sequencing. Results The mean E. coli count, as the most probable number, from the first river samples, taken on a falling tide on 23 March 2010, was 4.7?×?104/100 mL and 30 ciprofloxacin-resistant colonies were isolated. Twenty of the 30 colonies belonged to clone ST131; 10 of these had blaCTX-M-14 whereas the remaining 10 lacked ESBLs. The ST131 isolates represented two different PFGE types. No ciprofloxacin- or cefpodoxime-resistant E. coli were isolated from the second river sample taken at low tide. CTX-M-15, the most common ESBL in clinical E. coli, was not detected in the river samples. Conclusions Water from the River Thames in West London is contaminated, perhaps transiently, with antibiotic-resistant E. coli belonging to the clinically important O25b:H4-ST131 lineage.

Item Type: Article
Uncontrolled Keywords: anti-bacterial agents,bacterial typing techniques,drug resistance, bacterial,electrophoresis, gel, pulsed-field,escherichia coli,fluoroquinolones,humans,london,microbial sensitivity tests,molecular typing,plasmids,polymerase chain reaction,rivers,beta-lactamases,beta-lactams
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: Rhiannon Harvey
Date Deposited: 13 Jul 2011 11:04
Last Modified: 21 Apr 2020 16:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/33601
DOI: 10.1093/jac/dkq472

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