Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries

Choi, Seon Young, Jeon, Yoon-Seong, Lee, Je Hee, Choi, Boram, Moon, Sun Hwa, von Seidlein, Lorenz, Clemens, John D., Dougan, Gordon, Wain, John, Yu, Jun, Lee, Je Chul, Seol, Sung Yong, Lee, Bok Kwon, Song, Jae-Hoon, Song, Manki, Czerkinsky, Cecil, Chun, Jongsik and Kim, Dong Wook (2007) Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries. Journal of Medical Microbiology, 56 (11). pp. 1460-1466. ISSN 0022-2615

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The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established. EIEC, enteroinvasive E. coliMLST, multilocus sequence typingST, sequence type The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108). A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018)
Depositing User: Rhiannon Harvey
Date Deposited: 12 Jul 2011 10:30
Last Modified: 24 Oct 2022 01:10
DOI: 10.1099/jmm.0.47322-0

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