Effect of subinhibitory concentrations of antibiotics on mutation frequency in Streptococcus pneumoniae

Henderson-Begg, S. K., Livermore, DM and Hall, L. M. C. (2006) Effect of subinhibitory concentrations of antibiotics on mutation frequency in Streptococcus pneumoniae. Journal of Antimicrobial Chemotherapy, 57 (5). pp. 849-854. ISSN 0305-7453

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Abstract

Objectives: To investigate the effect of subinhibitory concentrations of ciprofloxacin, streptomycin, trimethoprim, ampicillin and erythromycin on mutation frequency in Streptococcus pneumoniae. Methods: Frequency of mutation to rifampicin resistance was determined in three clinical isolates grown with or without antibiotic treatment. dinB was analysed using PCR and DNA sequence determination. Results: Subinhibitory levels of ciprofloxacin and streptomycin increased the frequency of mutation to rifampicin resistance between 2- and 5-fold for all three isolates, which is comparable to the increase seen in mismatch repair mutants of this species. These increases appeared not to be dependent on the function of the error-prone DNA polymerase encoded by dinB, since one of the isolates was a naturally occurring deletion mutant for this gene. Trimethoprim increased the mutation frequency for two isolates, but not the dinB mutant; ampicillin and erythromycin had no significant effect on mutation frequencies for any isolate. Conclusions: Exposure to quinolones and aminoglycosides at subinhibitory concentrations may result in increased mutability in pneumococci, as well as selecting for resistance per se.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: Rhiannon Harvey
Date Deposited: 11 Jul 2011 10:05
Last Modified: 21 Apr 2020 19:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/33471
DOI: 10.1093/jac/dkl064

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