Long-Term temperature acclimation of photosynthesis in steady-state cultures of the polar diatom Fragilariopsis cylindrus

Mock, Thomas ORCID: https://orcid.org/0000-0001-9604-0362 and Hoch, Nikolai (2005) Long-Term temperature acclimation of photosynthesis in steady-state cultures of the polar diatom Fragilariopsis cylindrus. Photosynthesis Research, 85 (3). pp. 307-317. ISSN 0166-8595

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Cultures of the obligate psychrophilic diatom Fragilariopsis cylindrus (Grunow) were grown for 4 months under steady-state conditions at -1°C and +7°C (50 µmol photons m-2 s-1) prior to measurements in order to investigate long-term acclimation of photosynthesis to both temperatures. No differences in maximum intrinsic quantum yield of PS II (F V/F M) and relative electron transport rates could be detected at either temperature after 4 months of acclimation. Measurements of photosynthesis (relative electron transport rates) vs. irradiance (P vs. E curves) revealed similar values for relative light utilization efficiency (a = 0.57 at -1°C, a = 0.60 at +7°C) but higher values for irradiance levels at which photosynthesis saturates (E K) at -1°C and, therefore, higher maximum photosynthesis (P MAX = 54 (relative units) at -1°C, P MAX = 49 at +7°C). Nonphotochemical quenching (NPQ) measurements at 385 µmol photons m-2 s -1 indicated higher (37%) NPQ for diatoms grown at -1°C compared to +7°C, which was possibly related to a 2-fold increase in the concentration of the pigment diatoxanthin and a 9-fold up-regulation of a gene encoding a fucoxanthin chlorophyll a,c-binding protein. Expression of the D1 protein encoding gene psbA was ca. 1.5-fold up-regulated at -1°C, whereas expression levels of other genes from Photosystem II (psbC, psbU, psbO), as well as rbcL, the gene encoding the Rubisco large subunit were similar at both temperatures. However, a 2-fold up-regulation of a plastid glyceraldehyde-P dehydrogenase at -1°C indicated enhanced Calvin cycle activity. This study revealed for the first time that a polar diatom could efficiently acclimate photosynthesis over a wide range of polar temperatures given enough time. Acclimation of photosynthesis at -1°C was probably regulated similarly to high light acclimation.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups: Faculty of Science > Research Groups > Environmental Biology
Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Depositing User: Rosie Cullington
Date Deposited: 01 Jul 2011 08:25
Last Modified: 13 Apr 2023 13:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/33136
DOI: 10.1007/s11120-005-5668-9

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