Menin interacts directly with the homeobox-containing protein Pem

Lemmens, Irma H., Forsberg, Lars, Pannett, Anna A. J., Meyen, Eva, Piehl, Fredrik, Turner, Jeremy J. O., Van de Ven, Wim J. M., Thakker, Rajesh V., Larsson, Catharina and Kas, Koen (2001) Menin interacts directly with the homeobox-containing protein Pem. Biochemical and Biophysical Research Communications, 286 (2). pp. 426-31. ISSN 0006-291X

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The tumour suppressor gene causing multiple endocrine neoplasia type 1 (MEN1) encodes a 610 amino acid protein, menin. In order to identify menin-interacting proteins we used a yeast two-hybrid assay to screen a 12.5-dpc mouse embryo library with partial menin encompassing amino acids 278 to 476. This identified a homeobox containing protein encoded by a placenta and embryonic expression gene, referred to as Pem. GST-pull-down and coimmunoprecipitation experiments confirmed the interaction. Both proteins colocalised predominantly in the nucleus but were occasionally also found in the cytoplasm. Furthermore, in situ hybridisation studies revealed similarities in their expression patterns in mouse embryos and adult tissues. In adult mice both Men1 and Pem yielded strong signals in testis, Sertoli cells and particularly in seminiferous tubules. Thus, our study has identified that menin interacts with Pem, and the high expression of these proteins in the testis suggests a role in spermatogenesis.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit
Depositing User: Rhiannon Harvey
Date Deposited: 08 Jun 2011 14:47
Last Modified: 11 Jan 2024 01:25
DOI: 10.1006/bbrc.2001.5405

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