Ralston, Stuart H, Afzal, Muhammad A, Helfrich, Miep H, Fraser, William D, Gallagher, James A, Mee, Andrew and Rima, Bert (2007) Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone. Journal of Bone and Mineral Research, 22 (4). pp. 569-577. ISSN 1523-4681

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Abstract

Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.

Item Type:	Article
Uncontrolled Keywords:	base sequence,bone and bones,dna primers,distemper virus, canine,humans,laboratories,leukocytes, mononuclear,measles virus,osteitis deformans,paramyxoviridae infections,paramyxovirinae,rna, viral,reverse transcriptase polymerase chain reaction,sensitivity and specificity
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	http://dx.doi.org/10.1359/jbmr.070103
Depositing User:	Rhiannon Harvey
Date Deposited:	08 Jun 2011 13:29
Last Modified:	21 Apr 2020 19:15
URI:	https://ueaeprints.uea.ac.uk/id/eprint/32068
DOI:	10.1359/jbmr.070103

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