Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Ralston, Stuart H, Afzal, Muhammad A, Helfrich, Miep H, Fraser, William D, Gallagher, James A, Mee, Andrew and Rima, Bert (2007) Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone. Journal of Bone and Mineral Research, 22 (4). pp. 569-577. ISSN 1523-4681

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Abstract

Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.

Item Type: Article
Uncontrolled Keywords: base sequence,bone and bones,dna primers,distemper virus, canine,humans,laboratories,leukocytes, mononuclear,measles virus,osteitis deformans,paramyxoviridae infections,paramyxovirinae,rna, viral,reverse transcriptase polymerase chain reaction,sensitivity and specificity
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Rhiannon Harvey
Date Deposited: 08 Jun 2011 13:29
Last Modified: 21 Apr 2020 19:15
URI: https://ueaeprints.uea.ac.uk/id/eprint/32068
DOI: 10.1359/jbmr.070103

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