Identification of a periplasmic dimethylsulphoxide reductase in Hyphomicrobium EG grown under chemolithoheterotrophic conditions with dimethylsulphoxide as carbon source

Hatton, AD, Malin, G and McEwan, AG (1994) Identification of a periplasmic dimethylsulphoxide reductase in Hyphomicrobium EG grown under chemolithoheterotrophic conditions with dimethylsulphoxide as carbon source. Archives of Microbiology, 162 (1-2). pp. 148-150. ISSN 0302-8933

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Abstract

Hyphomicrobium EG can grow with dimethylsulphoxide as sole carbon and energy source with oxygen as electron acceptor. In the present work we have found that the dimethylsulphoxide reductase of this bacterium could be assayed with dithionite-reduced methylviologen as reductant but not with NADH. Sub-cellular fractionation of Hyphomicrobium EG showed that the dimethylsulphoxide reductase was a periplasmic enzyme. An antibody to the dimethylsulphoxide reductase of Rhodobacter capsulatus cross-reacted with a polypeptide in the periplasmic fraction from Hyphomicrobium EG which had the same M(r) as the dimethylsulphoxide reductase of Rhodobacter capsulatus. It is suggested that the reduction of dimethylsulphoxide in Hyphomicrobium involves respiratory electron transfer.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: Rosie Cullington
Date Deposited: 07 Jun 2011 07:41
Last Modified: 21 Apr 2020 21:34
URI: https://ueaeprints.uea.ac.uk/id/eprint/31898
DOI: 10.1007/s002030050117

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