Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages

Jofre-Monseny, Laia, de Pascual-Teresa, Sonia, Plonka, Eva, Huebbe, Patricia, Boesch-Saadatmandi, Christine, Minihane, Anne Marie ORCID: https://orcid.org/0000-0001-9042-4226 and Rimbach, Gerald (2007) Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. British Journal of Nutrition, 97 (05). pp. 864-871. ISSN 0007-1145

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Abstract

ApoE is secreted by macrophages at the lesion site of the atherosclerotic plaque, where it is thought to play a protective role against atherosclerosis independently of its effects on lipid metabolism. Of the three common isoforms for apoE, apoE4 is associated with higher risk of cardiovascular disease (CVD). In vitro studies have shown that recombinant apoE may act as an antioxidant in an isoform-dependent manner (E2>E3>E4). The oxidative status of the macrophages plays a key role in the process of atherosclerosis. In the present study the possible differential actions of apoE3 and apoE4 on several parameters of oxidative status were determined in stably transfected murine macrophages (RAW 264·7-apoE3 and -apoE4). No differences between genotypes were observed after peroxide challenge in either protection against cytotoxicity or in cell membrane oxidation, and modest differences were observed in the non-enzymatic antioxidants (glutathione and α-tocopherol) in apoE3 v. apoE4 macrophages. Importantly, cells secreting apoE4 showed increased membrane oxidation under basal conditions, and produced more NO and superoxide anion radicals than the apoE3 macrophages after stimulation. The present data suggest that apoE genotype influences the oxidative status of macrophages, and this could partly contribute to the higher CVD risk observed in apoE4 carriers.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	Rhiannon Harvey
Date Deposited:	31 May 2011 15:49
Last Modified:	19 Oct 2023 00:46
URI:	https://ueaeprints.uea.ac.uk/id/eprint/31640
DOI:	10.1017/S0007114507669219

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