Arabidopsis 14-3-3 lambda is a positive regulator of RPW8-mediated disease resistance

Yang, Xiaohua, Wang, Wenming, Coleman, Mark, Orgil, Undral, Feng, Jiayue, Ma, Xianfeng, Ferl, Robert, Turner, John G. and Xiao, Shunyuan (2009) Arabidopsis 14-3-3 lambda is a positive regulator of RPW8-mediated disease resistance. The Plant Journal, 60 (3). pp. 539-550. ISSN 1365-313X

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Abstract

The RPW8 locus from Arabidopsis thaliana Ms-0 includes two functional paralogous genes (RPW8.1 and RPW8.2) and confers broad-spectrum resistance via the salicylic acid-dependent signaling pathway to the biotrophic fungal pathogens Golovinomyces spp. that cause powdery mildew diseases on multiple plant species. To identify proteins involved in regulation of the RPW8 protein function, a yeast two-hybrid screen was performed using RPW8.2 as bait. The 14-3-3 isoform lambda (designated GF14?) was identified as a potential RPW8.2 interactor. The RPW8.2–GF14? interaction was specific and engaged the C-terminal domain of RPW8.2, which was confirmed by pulldown assays. The physiological impact of the interaction was revealed by knocking down GF14? by T-DNA insertion, which compromised basal and RPW8-mediated resistance to powdery mildew. In addition, over-expression of GF14? resulted in hypersensitive response-like cell death and enhanced resistance to powdery mildew via the salicylic acid-dependent signaling pathway. The results from this study suggest that GF14? may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Users 2731 not found.
Date Deposited: 23 May 2011 12:41
Last Modified: 21 Apr 2020 17:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/31255
DOI: 10.1111/j.1365-313X.2009.03978.x

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