Simple liquid chromatographic method for the determination of cefotaxime in human and rat plasma

Ling, Sharon Sheue Nee, Yuen, Kah Hay and Barker, Susan A. (2003) Simple liquid chromatographic method for the determination of cefotaxime in human and rat plasma. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 783 (1). pp. 297-301. ISSN 1570-0232

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A high-performance liquid chromatographic method with ultraviolet (UV) detection was developed for measuring cefotaxime in rat and human plasma. The method used direct injection of the plasma supernatant after deproteinization with 70% perchloric acid. Degradation of cefotaxime in acidic medium was retarded by adding phosphate buffer before centrifuging the sample. The mobile phase was 0.05 M aqueous ammonium acetate-acetonitrile-tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5. Analysis was run at a flow-rate of 1.0 ml/min, and a detection wavelength of 254 nm was used. The method has a quantification limit of 0.20 mug/ml. The within- and between-day coefficients of variation and accuracy values were less than 8% and 3%, respectively, while the recovery values were greater than 87% over the concentration range tested (0.20-50 mug/ml). The speed, sensitivity, specificity and reproducibility of this method make it particularly suitable for the routine determination of cefotaxime in human plasma. Moreover, only a relatively small sample plasma volume (100 mul) is required, allowing this method to be applied to samples taken from neonates. (C) 2002 Elsevier Science B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: cefotaxime,urine,cephalosporins,desacetylcefotaxime
Faculty \ School: Faculty of Science > School of Pharmacy
Depositing User: Rachel Smith
Date Deposited: 11 May 2011 11:02
Last Modified: 14 Aug 2023 14:30
DOI: 10.1016/S1570-0232(02)00657-8

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