Effect of plant-based feed ingredients on osmoregulation in the Atlantic salmon lens

Tröβe, Christiane, Rhodes, Jeremy D., Sanderson, Julie, Breck, Olav and Waagbø, Rune (2010) Effect of plant-based feed ingredients on osmoregulation in the Atlantic salmon lens. Comparative Biochemistry and Physiology - Part B Biochemistry & Molecular Biology, 155 (4). pp. 354-362.

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Abstract

Lenses of adult Atlantic salmon fed with a plant oil and plant protein-based diet (plant diet) were compared to lenses of fish fed a diet based on traditional marine ingredients (marine diet) with respect to biochemical composition and functionality ex vivo. After 12 months of feeding, plant diet-fed fish had smaller lenses with higher water contents and lower concentrations of histidine (His) and N-acetylhistidine (NAH) than fish fed with the marine diet. Cataract development in both dietary groups was minimal and no differences between the groups were observed. Lens fatty acid and lipid class composition differed minimally, although a significant increase in linoleic acid was observed. The lenses were examined for their ability to withstand osmotic disturbances ex vivo. Culture in hypoosmotic and hyperosmotic media led to increase and decrease of lens volume, respectively. Lenses from plant diet-fed fish were less resistant to swelling and shrinking, released less NAH into the culture medium, and accumulated His and NAH at higher rates than lenses from marine diet-fed fish. Culture in hypoosmotic medium resulted in higher cataract scores than in control and hyperosmotic medium. mRNA expression of selected genes, including glutathione peroxidase 4 and SPARC (secreted protein acidic and rich in cysteine), was affected by diet and osmotic treatment. It can be concluded that lenses of farmed Atlantic salmon are affected by the diet composition, both in biochemical composition and physiological functionality in relation to osmoregulation.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Pharmacy
Depositing User: Rachel Smith
Date Deposited: 07 Apr 2011 11:40
Last Modified: 21 Apr 2020 17:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/28410
DOI: 10.1016/j.cbpb.2009.12.002

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