Excited state dynamics in the green fluorescent protein

Tonge, Peter J. and Meech, Stephen R. ORCID: https://orcid.org/0000-0001-5561-2782 (2009) Excited state dynamics in the green fluorescent protein. Journal of Photochemistry and Photobiology A: Chemistry, 205 (1). pp. 1-11.

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The photophysical properties of the wild type green fluorescent protein (wtGFP), the isolated GFP chromophore in solution, and a number of GFP mutants are described. The mechanism of the very efficient radiationless decay of the isolated chromophore is discussed in the light of experimental measurements and theoretical calculations. The dramatic switch in photophysics, from radiationless decay dominated to proton transfer dominated, between the isolated chromophore and wtGFP is illustrated and discussed. The excited state dynamics and their manipulation through mutagenesis are described. The potential for utilizing GFP as a model system for studying the dynamics of proton transfer reactions in proteins, including low barrier hydrogen bonds and proton transport on proton wires, is discussed.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Depositing User: Rachel Smith
Date Deposited: 23 Mar 2011 12:59
Last Modified: 09 Feb 2023 13:35
URI: https://ueaeprints.uea.ac.uk/id/eprint/27009
DOI: 10.1016/j.jphotochem.2009.03.021

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