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Rushworth, Stuart, Macewan, David and O'Connell, Maria 
ORCID: https://orcid.org/0000-0002-0267-0951
(2008)
Lipopolysaccharide-induced expression of NAD(P)H:quinone oxidoreductase 1 and heme oxygenase-1 protects against excessive inflammatory responses in human monocytes.
Journal of Immunology, 181.
pp. 6730-6737.
ISSN 0022-1767
Abstract
Monocytes play a central role in the immunopathological effects of sepsis. This role is mediated by production of the cytokines TNF-a and IL-1ß. The transcription factor NF-E2-related factor 2 (Nrf2) regulates innate immune responses in various experimental disease models. Presently, the role of Nrf2-regulated genes in LPS-treated human monocytes is not well defined. Herein we show that Nrf2 mediates a significant regulation of LPS-induced inflammatory responses. Analysis of Nrf2-regulated gene expression in human monocytes showed that LPS induced the expression of the phase II detoxification gene NAD(P)H:quinone oxidoreductase 1 (NQO1). Furthermore, NQO1 mRNA or protein expression in response to LPS was regulated by Nrf2. Silencing Nrf2 expression in human monocytes inhibited LPS-induced NQO1 expression; however, in contrast, it significantly increased TNF and IL-1ß production. Silencing expression of NQO1 alone, or in combination with heme oxygenase-1 (HO-1) silencing, markedly increased LPS-induced TNF and IL-1ß expression. Additionally, overexpression of NQO1 and/or HO-1 inhibited LPS-induced TNF and IL-1ß expression. These results show for the first time that LPS induces NQO1 and HO-1 expression in human monocytes via Nrf2 to modulate their inflammatory responsiveness, thus providing novel potential therapeutic strategies for the treatment of sepsis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Science > School of Pharmacy |
| Depositing User: | Rachel Smith |
| Date Deposited: | 15 Mar 2011 16:45 |
| Last Modified: | 24 Oct 2022 01:04 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/26312 |
| DOI: |
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