A Tetranuclear Cu(I) Cluster in the Metallochaperone Protein CopZ

Hearnshaw, Stephen, West, Claire, Singleton, Chloe, Zhou, Liang, Kihlken, Margaret A., Strange, Richard W., Le Brun, Nick E. and Hemmings, Andrew M. (2009) A Tetranuclear Cu(I) Cluster in the Metallochaperone Protein CopZ. Biochemistry, 48 (40). pp. 9324-9326. ISSN 0006-2960

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Copper trafficking proteins and copper-sensitive regulators are often found to be able to bind multiple Cu(I) ions in the form of Cu(I) clusters. We have determined the high-resolution X-ray crystal structure of an Atx1-like copper chaperone protein from Bacillus subtilis containing a novel tetranuclear Cu(I) cluster. The identities and oxidation states of the cluster ions were established unambiguously by refinement of X-ray energy-dependent anomalous scattering factors. The [Cu4(S-Cys)4(N-His)2] cluster geometry provides new structural insights into not only the binding of multiple cuprous ions by metallochaperones but also protein-associated tetranuclear Cu(I) clusters, including those found in eukaryotic copper-responsive transcription factors.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
Depositing User: Users 2731 not found.
Date Deposited: 12 Sep 2011 09:51
Last Modified: 17 Sep 2020 23:34
URI: https://ueaeprints.uea.ac.uk/id/eprint/25387
DOI: 10.1021/bi9011995

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