Methylene blue as an electrochemical discriminator of single- and double-stranded oligonucleotides immobilised on gold substrates

Tani, Akiko, Thomson, Andrew J. and Butt, Julea (2001) Methylene blue as an electrochemical discriminator of single- and double-stranded oligonucleotides immobilised on gold substrates. The Analyst, 126 (10). pp. 1756-1759.

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Abstract

Self-assembly of thiol-terminated oligonucleotides on gold substrates provides a convenient and versatile route to DNA-functionalised surfaces. Here we show that the square-wave voltammetric peak position of methylene blue complexed to thiol-terminated single-stranded oligonucleotides immobilised on gold electrodes differs from that of methylene blue complexed to thiol-terminated double-stranded oligonucleotides immobilised on gold electrodes. The peak potential of methylene blue at the single-stranded oligonucleotide array was consistently found to occur at potentials ca. 10–15 mV more positive than that at double-stranded oligonucleotide arrays, the precise difference being dependent on the direction of the voltammetry. This voltammetric behaviour mirrors that found for methylene blue bound to freely diffusing single- and double-stranded calf thymus DNA and suggests that the immobilised oligonucleotides retain the methylene blue binding properties of their freely diffusing counterparts. Thus methylene blue provides a simple electrochemical indicator for the status of oligonucleotide-functionalised gold surfaces.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
Related URLs:
Depositing User: Rachel Smith
Date Deposited: 15 Feb 2011 13:13
Last Modified: 29 Aug 2019 13:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/21422
DOI: 10.1039/b104260m

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