Tethered DNA hairpins facilitate electrochemical detection of DNA ligation

Zauner, Gerhild, Wang, Yating, Lavesa-Curto, Manuel, MacDonald, Andrew, Mayes, Andrew G., Bowater, Richard P. ORCID: https://orcid.org/0000-0002-2745-7807 and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2005) Tethered DNA hairpins facilitate electrochemical detection of DNA ligation. The Analyst, 130 (3). pp. 345-349. ISSN 0003-2654

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Abstract

A novel electrochemical assay for DNA ligase activity is described. The assay exploits the properties of DNA hairpins tethered at one terminus to a gold electrode and labelled at the other with a ferrocene group for rapid characterisation of DNA status by cyclic voltammetry. Successful ligation of ‘nicked’ DNA hairpins is indicated by retention of the ferrocene couple when exposure to DNA ligase is followed by conditions that denature the hairpin. The results demonstrate the simplicity of integrating electrochemical detection with hairpin based biosensors and illustrate a new approach to the assay of DNA ligases, of which the NAD+-dependent enzymes represent a potential broad spectrum antibacterial drug target.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Chemistry of Materials and Catalysis Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017) Faculty of Science > Research Groups > Biosciences Teaching and Education Research Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017)
Depositing User:	Rachel Smith
Date Deposited:	17 Feb 2011 12:20
Last Modified:	22 Apr 2023 01:44
URI:	https://ueaeprints.uea.ac.uk/id/eprint/21412
DOI:	10.1039/b413556c

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