Tethered DNA hairpins facilitate electrochemical detection of DNA ligation

Zauner, Gerhild, Wang, Yating, Lavesa-Curto, Manuel, MacDonald, Andrew, Mayes, Andrew, Bowater, Richard P. and Butt, Julea (2005) Tethered DNA hairpins facilitate electrochemical detection of DNA ligation. The Analyst, 130 (3). p. 345. ISSN 0003-2654

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Abstract

A novel electrochemical assay for DNA ligase activity is described. The assay exploits the properties of DNA hairpins tethered at one terminus to a gold electrode and labelled at the other with a ferrocene group for rapid characterisation of DNA status by cyclic voltammetry. Successful ligation of ‘nicked’ DNA hairpins is indicated by retention of the ferrocene couple when exposure to DNA ligase is followed by conditions that denature the hairpin. The results demonstrate the simplicity of integrating electrochemical detection with hairpin based biosensors and illustrate a new approach to the assay of DNA ligases, of which the NAD+-dependent enzymes represent a potential broad spectrum antibacterial drug target.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
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Depositing User: Rachel Smith
Date Deposited: 17 Feb 2011 12:20
Last Modified: 21 Apr 2020 20:12
URI: https://ueaeprints.uea.ac.uk/id/eprint/21412
DOI: 10.1039/b413556c

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