DddQ, a novel, cupin-containing, dimethylsulfoniopropionate lyase in marine roseobacters and in uncultured marine bacteria

Todd, Jonathan D., Curson, Andrew R. J., Kirkwood, Mark, Sullivan, Matthew J. ORCID: https://orcid.org/0000-0003-2276-3132, Green, Robert T. and Johnston, Andrew W. B. (2011) DddQ, a novel, cupin-containing, dimethylsulfoniopropionate lyase in marine roseobacters and in uncultured marine bacteria. Environmental Microbiology, 13 (2). pp. 427-438. ISSN 1462-2920

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Abstract

Ruegeria (previously Silicibacter) pomeroyi DSS-3, a marine roseobacter, can catabolize dimethylsulfoniopropionate (DMSP), a compatible solute that is made in large amounts by marine plankton and algae. This strain was known to demethylate DMSP via a demethylase, encoded by the dmdA gene, and it can also cleave DMSP, releasing the environmentally important volatile dimethyl sulfide (DMS) in the process. We found that this strain has two different genes, dddP and dddQ, which encode enzymes that cleave DMSP, generating DMS plus acrylate. DddP had earlier been found in other roseobacters and is a member of the M24 family of peptidases. The newly discovered DddQ polypeptide contains a predicted cupin metal-binding pocket, but has no other similarity to any other polypeptide with known function. DddP- and DddQ- mutants each produced DMS at significantly reduced levels compared with wild-type R. pomeroyi DSS-3, and transcription of the corresponding ddd genes was enhanced when cells were pre-grown with DMSP. Ruegeria pomeroyi DSS-3 also has a gene product that is homologous to DddD, a previously identified enzyme that cleaves DMSP, but which forms DMS plus 3-OH-propionate as the initial catabolites. However, mutations in this dddD-like gene did not affect DMS production, and it was not transcribed under our conditions. Another roseobacter strain, Roseovarius nubinhibens ISM, also contains dddP and has two functional copies of dddQ, encoded by adjacent genes. Judged by their frequencies in the Global Ocean Sampling metagenomic databases, DddP and DddQ are relatively abundant among marine bacteria compared with the previously identified DddL and DddD enzymes.

Item Type: Article
Uncontrolled Keywords: sdg 14 - life below water ,/dk/atira/pure/sustainabledevelopmentgoals/life_below_water
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Users 2731 not found.
Date Deposited: 15 Feb 2011 11:37
Last Modified: 21 Nov 2022 16:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/21384
DOI: 10.1111/j.1462-2920.2010.02348.x

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