FTIR and solid state13C NMR spectroscopy of proteins of wet cooked and popped sorghum and maize

Duodu, K, Tang, H, Grant, A, Wellner, N, Belton, Peter and Taylor, JRN (2001) FTIR and solid state13C NMR spectroscopy of proteins of wet cooked and popped sorghum and maize. Journal of Cereal Science, 33 (3). pp. 261-269.

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Abstract

Fourier transform infrared (FTIR) and solid state13C NMR spectroscopic methods were used to investigate changes in maize and sorghum proteins on wet cooking and popping. FTIR spectra indicated that wet cooking led to proteins in two normal sorghums, namely NK 283 (a red hybrid) and KAT 369 (a white variety), two sorghum mutants (P850029 and P851171) and a maize hybrid (PAN 6043) assuming more antiparallel intermolecular ß-sheet character, possibly at the expense of some a-helical conformation. Solid state13C NMR, using the technique of Cross Polarisation Magic Angle Spinning showed shifts of the protein carbonyl carbon and a-carbon resonances upfield on wet cooking in all samples, also indicating a change in protein secondary structure from a-helical to ß-sheet conformation. The extent of secondary structural change on wet cooking seemed to be greater in sorghum than in maize and may have a bearing on the inferior protein digestibility of wet cooked sorghum compared to maize. Popping produced the same secondary structural change as observed for wet cooking in both sorghum and maize. However, the extent of change on popping was less than on wet cooking in sorghum and maize.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017)
Depositing User:	Rachel Smith
Date Deposited:	22 Feb 2011 14:58
Last Modified:	15 Dec 2022 01:48
URI:	https://ueaeprints.uea.ac.uk/id/eprint/21277
DOI:	10.1006/jcrs.2000.0352

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