Escherichia coli cytochrome c nitrite reductase NrfA

Clarke, Thomas A. ORCID: https://orcid.org/0000-0002-6234-1914, Mills, Paul C., Poock, Susie R., Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226, Cheesman, Myles R., Cole, Jeffrey A., Hinton, Jay C. D., Hemmings, Andrew M., Kemp, Gemma, Söderberg, Christopher A. G., Spiro, Stephen, van Wonderen, Jessica and Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 (2008) Escherichia coli cytochrome c nitrite reductase NrfA. Methods in Enzymology, 437. pp. 63-77. ISSN 1557-7988

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Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory (former - to 2025) Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Light and Energy (former - to 2025) Faculty of Science > Research Groups > Chemistry of Life Processes (former - to 2025) Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology Faculty of Science > Research Groups > Pharmacology and Biological Chemistry
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:36
Last Modified:	18 Jun 2026 15:15
URI:	https://ueaeprints.uea.ac.uk/id/eprint/191
DOI:	10.1016/S0076-6879(07)37004-3

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