Clarke, Thomas A., Mills, Paul C., Poock, Susie R., Butt, Julea N., Cheesman, Myles R., Cole, Jeffrey A., Hinton, Jay C. D., Hemmings, Andrew M., Kemp, Gemma, Söderberg, Christopher A. G., Spiro, Stephen, Van Wonderen, Jessica and Richardson, David J. (2008) Escherichia coli cytochrome c nitrite reductase NrfA. Methods in Enzymology, 437. pp. 63-77. ISSN 1557-7988

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Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:36
Last Modified:	17 Sep 2020 23:37
URI:	https://ueaeprints.uea.ac.uk/id/eprint/191
DOI:	10.1016/S0076-6879(07)37004-3

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