Escherichia coli cytochrome c nitrite reductase NrfA

Clarke, Thomas A., Mills, Paul C., Poock, Susie R., Butt, Julea N., Cheesman, Myles R., Cole, Jeffrey A., Hinton, Jay C. D., Hemmings, Andrew M., Kemp, Gemma, Söderberg, Christopher A. G., Spiro, Stephen, van Wonderen, Jessica and Richardson, David J. (2008) Escherichia coli cytochrome c nitrite reductase NrfA. Methods in Enzymology, 437. pp. 63-77. ISSN 1557-7988

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Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:36
Last Modified:	19 May 2025 08:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/191
DOI:	10.1016/S0076-6879(07)37004-3

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