The role of multihaem cytochromes in the respiration of nitrite in Eschericia coli and fe(III) in Shewanella oneidensis

Clarke, Thomas A. ORCID: https://orcid.org/0000-0002-6234-1914, Holley, Tracey, Hartshorne, Robert S., Fredrickson, Jim K., Zachara, John M., Shi, Liang and Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 (2008) The role of multihaem cytochromes in the respiration of nitrite in Eschericia coli and fe(III) in Shewanella oneidensis. Biochemical Society Transactions, 36 (5). pp. 1005-1010. ISSN 1470-8752

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Abstract

The periplasmic nitrite reductase system from Escherichia coli and the extracellular Fe(III) reductase system from Shewanella oneidensis contain multihaem c-type cytochromes as electron carriers and terminal reductases. The position and orientation of the haem cofactors in multihaem cytochromes from different bacteria often show significant conservation despite different arrangements of the polypeptide chain. We propose that the decahaem cytochromes of the iron reductase system MtrA, MtrC and OmcA comprise pentahaem ‘modules’ similar to the electron donor protein, NrfB, from E. coli. To demonstrate this, we have isolated and characterized the N-terminal pentahaem module of MtrA by preparing a truncated form containing five covalently attached haems. UV–visible spectroscopy indicated that all five haems were low-spin, consistent with the presence of bis-His ligand co-ordination as found in full-length MtrA.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Energy Materials Laboratory
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Organisms and the Environment
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 13:36
Last Modified: 16 May 2023 00:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/186
DOI: 10.1042/BST0361005

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