Inducible gene expression in transient transgenic Xenopus embryos

Wheeler, Grant N., Lavery, Danielle L. and Hoppler, Stefan (2008) Inducible gene expression in transient transgenic Xenopus embryos. Methods in Molecular Biology, 469. pp. 431-449. ISSN 1940-6029

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Abstract

Xenopus laevis has for many years been successfully used to study Wnt signaling during early development. However, because loss of function and gain of function experiments generally involve injecting RNA, DNA, or morpholinos into early embryos (1- to 32-cell), major phenotypes are often observed before the embryo has reached later stages of development. The combined use of transgenics and a heat shock inducible system has overcome these problems and enables investigations of Wnt signaling at later stages of Xenopus embryonic development, including organogenesis.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Wheeler Group Faculty of Science > Research Groups > Cells and Tissues
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:36
Last Modified:	28 Mar 2025 03:41
URI:	https://ueaeprints.uea.ac.uk/id/eprint/160
DOI:	10.1007/978-1-59745-249_27

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