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Ivory, K, Sarria, B, Fairweather-Tait, S J 
ORCID: https://orcid.org/0000-0002-1413-5569 and Hughes, David
(2007)
Reticulated platelets interfere with flow cytometric reticulocyte counts.
International Journal of Laboratory Hematology, 29 (5).
pp. 352-360.
ISSN 1751-5521
Abstract
As part of an iron absorption study, we needed to accurately count reticulocytes in the peripheral blood of healthy human volunteers before measuring their enrichment with stable iron isotopes given in an oral dose. Recent studies have suggested the usefulness of reticulocyte counting by flow cytometry, through a combination of differential light scatter and measurement of the stoichiometric binding of thiazole orange (TO) to RNA within the maturing erythrocyte. Using this method we set out to improve the precision of our quantitative analysis by counting more cells, as reticulocytes normally comprise
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | blood platelets,cell separation,flow cytometry,humans,image processing, computer-assisted,reticulocyte count,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | EPrints Services |
| Date Deposited: | 01 Oct 2010 13:38 |
| Last Modified: | 24 Oct 2022 01:23 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/1526 |
| DOI: | 10.1111/j.1365-2257.2006.00881.x |
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