Reticulated platelets interfere with flow cytometric reticulocyte counts

Ivory, K, Sarria, B, Fairweather-Tait, S J ORCID: https://orcid.org/0000-0002-1413-5569 and Hughes, D. A. (2007) Reticulated platelets interfere with flow cytometric reticulocyte counts. International Journal of Laboratory Hematology, 29 (5). pp. 352-360. ISSN 1751-5521

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Abstract

As part of an iron absorption study, we needed to accurately count reticulocytes in the peripheral blood of healthy human volunteers before measuring their enrichment with stable iron isotopes given in an oral dose. Recent studies have suggested the usefulness of reticulocyte counting by flow cytometry, through a combination of differential light scatter and measurement of the stoichiometric binding of thiazole orange (TO) to RNA within the maturing erythrocyte. Using this method we set out to improve the precision of our quantitative analysis by counting more cells, as reticulocytes normally comprise

Item Type:	Article
Uncontrolled Keywords:	blood platelets,cell separation,flow cytometry,humans,image processing, computer-assisted,reticulocyte count,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:38
Last Modified:	02 Feb 2023 10:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/1526
DOI:	10.1111/j.1365-2257.2006.00881.x

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