Evidence for GC preference by monocot Dicer-like proteins

Ho, Thien, Rusholme Pilcher, Rachel L., Edwards, Mary-Lou, Cooper, Ian, Dalmay, Tamas and Wang, Hui (2008) Evidence for GC preference by monocot Dicer-like proteins. Biochemical and Biophysical Research Communications, 368 (2). pp. 433-437. ISSN 1090-2104

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Abstract

Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 13:36
Last Modified: 21 Apr 2020 17:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/142
DOI: 10.1016/j.bbrc.2008.01.110

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